Protein conversion into gene & NCBI BLAST on it

Bioinformatics is the subject of today's science. Various Bioinformatics tools are available online. Scholars like to search mainly  NCBI blastp, NCBI blastn, NCBI ftp site, NCBI nucleotide blast, primer blast NCBI, blast 2, blast search, and various other keywords related to Bioinformatics tools.
In this post, you will learn:

• How to download/copy genome sequence 
• How to change genome format (FASTA Format)
• How to convert protein into the nucleotide sequence 
• How to do Blast of the nucleotide sequence 

Let us discuss them one by one.

   Download genome sequence 

   Search NCBI on the google page.

   Click on National Center for Biotechnology Information

   Select gene/protein from “all database" option

   Write the name of the protein/gene on the box and click on "Search" as shown above. All papers related to protein/gene will be open.

                     Play game: Magic Ball 

Click on the protein of the desired organism (complete paper of that organism will appear).

Scroll down and copy this sequence under the heading of “origin” by pressing Ctrl+C.
(don’t copy digits and //)

Change format of genome sequence

Open a new tab and write “sequence format conversion”
Open the link shown below.

Paste the sequence in the box by pressing Ctrl+V.

Click on output format and choose“FASTA format” from the list and click on Submit

Wait for a while, complete sequence, with the symbol of “>” will appear.

Conversion of the Protein into the Nucleotide 

Copy that sequence by pressing Ctrl+C.                                                                   

In new tab write “SEQUENCE TRANSLATOR TOOL”

Click on the link shown.

                                                                                

To convert protein sequence into nucleotide sequence, click on “Launch Backtranseq”

Paste the sequence in the box by pressing Ctrl+V. Select required species from CODON USAGE TABLE and click submit.

Protein sequence is converted into Gene sequence.

Nucleotide sequence BLAST

Now Copy that nucleotide sequence.

In the new tab re-open, NCBI. Click on National Center for Biotechnology Information.

Click on Resource List (A-Z)

Click on Basic Local Alignment Search Tool (BLAST)

Click on Nucleotide Blast

Paste the sequence and click on Blast.

Graphic Summary, of the genome sequence, will appear.

This compares our query data with standard.