Bioinformatics is the subject of today's science. Various Bioinformatics tools are available online. Scholars like to search mainly NCBI blastp, NCBI blastn, NCBI ftp site, NCBI nucleotide blast, primer blast NCBI, blast 2, blast search, and various other keywords related to Bioinformatics tools.
In this post, you will learn:
- How to Convert nucleotide sequence into protein
- How to perform BLAST on protein
Write
NCBI in google box.
Click on National Center for Biotechnology
Information
From all database choose gene.
Write name of gene in the box and click on search.as shown below.
From all database choose gene.
Write name of gene in the box and click on search.as shown below.
List
of papers will be open.
Choose one paper, complete paper will open.
Scroll down and click
on “FASTA.” Sequence
will be change in FASTA format.
Copy
this sequence by pressing Ctrl+C.
In
new tab write “sequence format conversion” and open the link shown.
Choose DNA and paste
the sequence in box by pressing Ctrl+V.
Now, scroll down, click
on output format, choose “FASTA format” from the box and click on submit.
Complete sequence, with symbol of “>” will appear.
Copy that sequence by pressing Ctrl+C.
In new tab write “SEQUENCE TRANSLATOR TOOL”
Click on the link shown.
heading of Nucleotide Sequence
Translation as shown:
Paste the sequence in box by pressing Ctrl+V and Click
on SUBMIT.
Nucleotide sequence has been converted into protein
Now Copy that aminamino acid sequence.
In the new tab re-open, NCBI. Click on National Center for Biotechnology Information.
Click on Resource List (A-Z)
Click on Basic Local Alignment Search Tool (BLAST)
Click on Nucleotide Blast
Paste the sequence and click on Blast.
Graphic Summary, of the protein sequence, will appear.
This compares our query data with standard.
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